Nitrogen-induced metabolic changes and molecular determinants of carbon allocation in Dunaliella tertiolecta
نویسندگان
چکیده
منابع مشابه
Nitrogen-induced metabolic changes and molecular determinants of carbon allocation in Dunaliella tertiolecta
Certain species of microalgae are natural accumulators of lipids, while others are more inclined to store starch. However, what governs the preference to store lipids or starch is not well understood. In this study, the microalga Dunaliella tertiolecta was used as a model to study the global gene expression profile regulating starch accumulation in microalgae. D. tertiolecta, when depleted of n...
متن کاملIsolation of Intact Chloroplasts from Dunaliella tertiolecta.
Cells of Dunaliella tertiolecta from the log phase of growth were broken by rapid extrusion at low pressure through a Yeda press and the chloroplasts were isolated by centrifugation through a Percoll gradient. Osmolarity of the growth media, the suspending media, and the Percoll gradient was kept identical to minimize change in chloroplast volume and mitochondrial entrapment. The isolated intac...
متن کاملCharacterization and expression of AMP-forming Acetyl-CoA Synthetase from Dunaliella tertiolecta and its response to nitrogen starvation stress
AMP-forming acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA. Here, a cDNA of ACS from Dunaliella tertiolecta (DtACS) was isolated using RACEs. The full-length DtACS cDNA (GenBank: KT692941) is 2,464 bp with a putative ORF of 2,184 bp, which encodes 727 amino acids with a predicted molecular weight of 79.72 kDa. DtACS has a close relationship with Chlamydomonas reinhardtii and ...
متن کاملEffects of Light Intensity and Nitrogen Starvation on Glycerolipid, Glycerophospholipid, and Carotenoid Composition in Dunaliella tertiolecta Culture
Time-course variation of lipid and carotenoid production under high light (300 μE/m²s) and nitrogen starvation conditions was determined in a Dunaliella tertiolecta strain. Nanoelectrospray (nanoESI) chip based direct infusion was used for lipid analysis and ultra-performance liquid chromatography (UPLC) coupled with a photodiode array (PDA) or atmospheric chemical ionization mass spectrometry ...
متن کاملThe effect of carbon dioxide concentration and buffer system on nitrate and nitrite assimilation by Dunaliella tertiolecta.
Dunaliella tertiolecta required carbon dioxide in substrate concentrations (1.75 %, v/v) to assimilate either nitrate or nitrite at maximum rates in light. The addition of glucose, glycerol, acetate, pyruvate or a-ketoglutarate did not remove the requirement for carbon dioxide. The rates of nitrate and nitrite assimilation in light depended upon the buffer system used. The lowest rates of nitra...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Scientific Reports
سال: 2016
ISSN: 2045-2322
DOI: 10.1038/srep37235